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Stellate Cells, Human Cryopreserved, 0.2 Million Cells

Stellate Cells, Human Cryopreserved, 0.2 Million Cells

Catalog #: HUCLS-200K

Low passage stellate cells dervied from human liver tissue containing 200,000 cells/vial.

Do you need a modified version of this cell product, such as different vialing sizes, or isolations from special donors or species? Contact Lonza CellBio Services to discuss custom isolation, expansion, and testing options for your research

 
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 831.00 CHF
AMP

Product Overview

Stellate cells are a resident cell type of the liver primarily functioning to store retinoids. In response to liver damage, stellate cells rapidly lose the stored retinoids and differentiate into a proliferating fibroblast-like cell that begins depositing collagen matrix. This activity causes buildup of collagen in the liver eventually leading to cirrhosis.

Stellate cells can be isolated from liver tissue, enriched, and placed into cell culture. When placed into culture, stellate cells begin to immediately differentiate into the activated state, characterized by high expression of alpha-smooth muscle actin, a-SMA.

Human Stellate Cells are stellate cells enriched from human liver isolations and grown to >80% confluency and then cryopreserved.

Human Stellate Growth Medium (Catalog MST-500BK) is available to support optimal culture health and growth characteristics of stellate cells.

Characterization performed:

  • Post-thaw plating to observe characteristic stellate morphology.
  • Flow cytometry using antibodies for Vimentin Immunocytochemistry for a-SMA

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Benefits

 Low passage for high activation potential

Characterized for alpha-SMA

 

Applications

  • ADMETox
  • Disease research

Storage and Content

Cryopreserved Cells, 200,000 viable cells
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SDS, CoA, and Instructions

Certificate of Analysis (CoA)

Please enter Lot Number, including all zeros, located on the product label and please take into account that it is case sensitive.

  • Instructions for Use - Hepatocyte and Non-Parenchymal Cell (NPC) 2D Co-Culture

    Protocol for 2D co-culture of human hepatocytes and non-parenchymal cells
  • Hepatocytes and Non-parenchymal Cells - Development and Optimization of a Comprehensive Co-Culture

    Tech Note detailing the development of a co-culture protocol for Heps and NPCs
  • Instructions - Cryopreserved Human Stellate Cells

    Technical Instructions for culturing human stellate cells
  • 3D Spheroid Primary Human Hepatocyte and Non-Parenchymal Cell (NPC) Co-Culture - Instructions for use

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Educational Material

Brochures, White Papers etc.

  • Primary Human Hepatocyte 3D Spheroids Co-cultured with Non-Parenchymal Cells

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Human Hepatocte Inventory

View donor information and select characteristics of currently available hepatocyte lots.

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